Anti-Ig second step staining

Three options on the Stain sets control panel, If checked, enable anti-Ig 2nd step staining.   

Unconjugated puts on your reagent pick tree purified antibodies with no conjugates or handles. 

Anti-Ig puts on the tree reagents that detect immunoglobulins.

Allow anti-Ig cross reactions? suspends CytoGenie's specificity protection for your current stain set.  To understand the risks in doing this, click here.

If you opt to allow anti-Ig cross reactions, a Caution window will appear warning that you may be running a risk.  Click Review risk.  Click here to read the text .

If you want to retain Cyto Genie's specificity protection, return to the Caution window and click Cancel.  If you want to relax the protection for this stain set, click Accept risks.

On the Reagent pick tree, click to select any unconjugated reagent.

If you have not previously clicked on the Control panel to allow anti-Ig cross reactions for this stain set, a message will appear in place of the Reagent pick tree.  If you have, the message will not appear.

Either way, the pop-up window will appear and give you a choice.  Do you want to Select a 2nd step? for the unconjugated reagent you have just selected.  Or would you prefer No 2nd step (do not detect 1st step).  Click to check one of the two round buttons.

If you chose No 2nd step, the window will close and you will return to the Stain sets, which will display the unconjugated reagent you picked.  If you chose to Select a 2nd step, go to the list below and click the reagent you want to use.

Once you select the 2nd step reagent, your stain set will now display it along with the unconjugated reagent you picked.  Both will show the same color conjugate.  You may continue picking reagents, including other 2nd step anti-Ig reagents, until you complete your stain set.
 

Pick an instrument configuration

Reuse or modify earlier stain sets

Use control panel to customize your reagent pick tree

Make your picks

Learn more about your stain sets

Add information to your stain set

Favor specificity

Gene expression reporters

Add a special reagent

Specify dilutions & staining steps