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Create a new DiVa template If you use BD’s DiVa software to control a flow cytometry instrument, CytoGenie Pro and CytoGenie Lab can create templates to export your protocol to any configuration of that instrument. CytoGenie Basic does not allow youto do this. To store the structure of your flow cytometry experiments, BD’s DiVa software uses specialized files, which contain instrument settings, global worksheets used for calibration and other information. CytoGenie can use one of these files as a template to generate protocols for your experiment.  Launch the DiVa software on each flow instrument for which you wish to create a template in CytoGenie. (The DiVa files can be machine specific.) On the DiVa screen:
- Locate an existing experiment similar to the one you want to create
- Right-click on the experiment name. A menu will pop up.
- Click Export. A sub-menu will appear.
- Click Experiment Template.
DiVa will then ask a series of questions. Ignore them. Just click Next and finally OK. DiVa will save the template to the folder BDExport, which is generally located in the root directory C:\BDExport. Look in BD Export folder > Templates > Experiments,and find the file with the experiment name you selected in Step 2above. It will have the file extension .xml. Click to select. If you don’t find the folder there, ask your system administrator. 
Return to CytoGenie. If the Instruments tab is not visible on the same line as Protocols and Reagents, click View. 
A menu will drop down. Click Instruments.  The Instrument configurations pick list will appear. Click the Downward options arrow.
An Options menu will pop up. If Edit in place is unchecked, leave it. If it is checked, click to uncheck it.  On the Instrument configurations pick list, click New.  A Diva template entry form will appear. Click Pick.
The Instrument configuration pick list will reapear. Click your configuration. Click Pick.  The Instrument configuration window will appear. If you want a DiVa template for this configuration, click Done.
The entry form will reappear with the configuration you picked. Double-click anywhere in the DiVa template field.  An input window will appear. Enter a name for your template. Click OK.
A window will display the DiVa exports folder. Click on the xml file that you previously exported from DiVa. Click Use.
 If a fluorochrome from the DiVa file differs from our nomenclature,CytoGenie will prompt you with its name and ask you to choose analternative. For each unrecognized fluorochrome, click the appropriatechoice.
Once you’ve cleared up these confusions, a summary screen will appear,telling you where CytoGenie has stored different parts of thetemplate. Click OK and Save.  The next time you exit CytoGenie, the program will ask if you want to Save to Server. You must do so to make the new configuration available to other users. If you wish to keep it only for yourself, click the File tab before exiting.
 A menu will drop down. Click Save knowledge base locally.
You should now be able to export your files to DiVa. If you encounter any problems exporting, please Ask the Genie Online. Please remember: You may export to instruments only with CytoGenie Pro and CytoGenie Lab.
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