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Use control panel to customize your reagent pick tree
 To speed up your work and make it easier for you to see what you're doing, CytoGenie gives you several newly developed ways to focus on just the reagents you need. Click Control panel.
 The Control panel will appear with different options selected depending on previous use. Click the window header and drag it to a convenient spot on top of all your other windows. For your convenience, the Control panel will remain visible until you minimize it or click Done.
At any time, you may click to check or uncheck any of the boxes or to make selections on the drop-down menus. Then click Apply.
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If you click to select this:
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Your reagent pick tree will do this:
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Match to instrument |
Display only reagents your selected instrument will detect
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Favorites only
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Display only reagents you put on your Favorites list |
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Isotype controls
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Include antibody reagents that behave in a "pre-immune" manner
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Sources (Check at least one or no reagents will display)
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Invitrogen
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Include reagents from the Invitrogen catalog, whether or not you normally keep them in your inventory. (CytoGenie enables you to buy any of these online.)
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Other sources
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Include self-prepared reagents or those from other companies
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Local stock (Check at least one or no reagents will display)
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Available
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Include reagents currently in your inventory
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Need to buy/prepare
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Include reagents normally in your inventory but currently depleted and those you keep listed in your virtual inventory
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Antibodies
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Conjugated
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Include purified antibodies with conjugates or handles
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* Unconjugated
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Include purified antibodies with no conjugates or handles, used with anti-Ig 2nd step stains
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* Anti-Ig
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Include reagents that detect immunoglobulins |
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* Allow anti-Ig cross reactions?
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Suspends CytoGenie's specificity protection for your current stain set. This allows you to pick 2nd step anti-Ig stains that might cause unintended reactions and errors in reading your results.
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Species
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Target species
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Include reagents that target selected species
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Show antibodies reactive with
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Include antibodies that react with selected species
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* Click here for more on Anti-Ig 2nd step staining.
Add more species
 If you want the pick tree to show antibodies that react with multiple species, click Add more species.
 The Control panel will display a list of species. Click to pick those you want. Your pick tree will show antibodies that react with the species you pick.
Favorites
 The Control panel also gives you other powerful choices. To create a list of your favorite reagents, go to Favorites only and click Pick.
 The Organize favorites window will appear. Click to highlight the entire tree. Click & drag the scroll bar to view the entire list. Click the boxes in the Favorite column of those reagents you want to put on your Favorites list. When you return to the Control panel, click to check Favorites only. Your tree and table will now show only those reagents you have put on your Favorites list.
Premixes
 To activate the Premix pick list, click Show premixes.
 The pick list will appear. Click to check the premix box. Click to highlight the premix you want in your stain set. Click Pick.
 Your premix will appear in your stain set.
Stain set functions
 For further options, click Stain set functions.
 A sub-menu will appear. Click to highlight one or more conjugated reagents on your Stain set window. Click See fluorochrome spectra.on the Control panel.
A window will display the emissions and excitations profiles for the highlighted reagents.
To boost the visibility of dull antigens to overcome possible interference from others, click Favor specificity. Click here for more information on how to make this work.
When you've finished picking your stain set(s), click Specify dil'n/steps to set the values you want. Click here for the how-to.
Pick an instrument configuration
Reuse or modify earlier stain sets
Make your picks
Anti-Ig 2nd step staining
Learn more about your stain sets
Add information to your stain set
Favor specificity
Gene expression reporters
Add a special reagent
Specify dilutions & staining steps
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